The following is précised from García-Fiñana et al. (2003).
The volume of an object can be expressed as
$$ V = \int_{a}^{b} A(x) \mathrm{d}x, $$
where \(A(x)\), called the area function, denotes the intersection area between the object and a plane normal to a fixed, conveniently oriented sampling axis, at a point of abscissa \(x.\) \(A(x)\) is bounded and integrable in a bounded domain \([a, b]\), which represents the orthogonal linear projection of the object on the sampling axis.
To estimate \(V\) intersect the object by a series of parallel planes a distance \(T\) apart, to produce \(n\) section areas \({A_1, A_2, ..., A_n}\). More precisely, the \(i\)th section area is \(A_i = A(z + (i - 1)T)\), \(i = 1, 2, ..., n\), where \(z\) is a uniform random abscissa in the interval \([a, a + T)\). The latter condition ensures that
$$ \hat{V} = T \cdot (A_1 + A_2 + ... + A_n) $$
is an unbiased estimator of \(V\) irrespective of the shape of the object.
The section areas can be estimated via point counting (Mathieu et al. 1981). A convenient test system is a square grid of side \(u\). The grid is superimposed uniformly at random on each section image. Let \(P_i\) denote the number of test points hitting a section of unknown area \(A_i\). An unbiased estimator of \(A_i\) is \(\hat{A_i} = u^2 P_i\), whereby
$$ \tilde{V} = T \cdot u^2 \cdot (P_1 + P_2 + ... + P_n) $$
is an unbiased estimator of \(V\) based on two sampling stages, namely Cavalieri sectioning and point counting.
From Gundersen et al. (1999), Kiêu et al. (1999), García-Fiñana (2000), García-Fiñana and Cruz-Orive (2000a,b), it can be shown that
$$ \mathrm{CE}^2(\tilde{V}) = \mathrm{CE}^2(\hat{V}) + \mathrm{CE}^2_{PC}(\tilde{V}), $$
where \(\mathrm{CE}^2(\hat{V})\) is the true contribution of the variability among sections and \(\mathrm{CE}^2_{PC}(\tilde{V})\) is the true mean variability due to point counting within sections.
The estimator of each term in the previous equation from the observed data is denoted in lower case, namely
$$ \mathrm{ce}^2(\tilde{V}) = \mathrm{ce}^2(\hat{V}) + \mathrm{ce}^2_{PC}(\tilde{V}). $$
From Cruz-Orive (1999, equation 2.7), the sections and point counting contributions may be predicted as
where
$$ \sum P_i = P_1 + P_2 + ... + P_n $$
and
$$ C_k = \sum^{n - k}_{i = 1} P_i P_{i+k}, k = 0, 1, ..., n - 1. $$
The numerical coefficient \(\alpha(q)\), updated in García-Fiñana and Cruz-Orive (2000b, equation 26b), depends on the fractional smoothness constant \(q\) of the area function and has the expression
where \(q \in [0, 1] \) and \(\Gamma()\) and \(\zeta()\) denote gamma and Riemann zeta functions respectively.
From a sample of at least five sections, the only estimator of \(q\) available at the time of writing is Kiêu-Souchet’s described in Kiêu et al. (1999). Namely,
For \(n < 5\), \(q\) can be set equal to 1 for fairly regular, quasi-ellipsoidal objects and 0 for irregular objects.
Finally, as described in Matheron (1971) and Gundersen and Jensen (1987), \(\hat{v}\) is an estimator of the point counting or nugget variance within sections. Namely,
$$ \hat{v} = 0.0724 \cdot (\overline{B} / \sqrt{\overline{A}}) \cdot (n \cdot \sum P_i)^{1/2}, $$
where \(\overline{B}\), \(\overline{A}\) are estimates of the mean boundary length and the mean area of the sections, respectively. Thus, \(\overline{B} / \sqrt{\overline{A}}\) is a dimensionless shape coefficient of the sections and, for practical purposes, may be estimated from a few sections.
CRUZ-ORIVE, L.M., Precision of Cavalieri sections and slices with local errors. J. Microsc. 193, 182–198 (1999).
GARCÍA-FIÑANA, M. Muestreo Sistemático en R. Aplicaciones a la Estereología, Tesis doctoral. Universidad de Cantabria (2000).
GARCÍA-FIÑANA, M., CRUZ-ORIVE, L.M., New approximations for the efficiency of Cavalieri sampling. J. Microsc. 199, 224–238 (2000a).
GARCÍA-FIÑANA, M., CRUZ-ORIVE, L.M., Fractional trend of the variance in Cavalieri sampling. Image Anal. Stereol. 19, 71–79 (2000b).
GARCÍA-FIÑANA, M., CRUZ-ORIVE, L. M., MACKAY, C.E., PAKKENBERG, B., ROBERTS, N. Comparison of MR imaging against physical sectioning to estimate the volume of human cerebral compartments. NeuroImage, 18, 505-516 (2003).
GUNDERSEN, H.J.G., JENSEN, E.B., The efficiency of systematic sampling in stereology and its prediction. J. Microsc., 147, 229–263 (1987).
GUNDERSEN, H.J.G., JENSEN, E.B.V., KIÊU, K., NIELSEN, J. The efficiency of systematic sampling in stereology - Reconsidered. J. Microsc., 193, 199–211 (1999).
KIÊU, K., SOUCHET, S., ISTAS, J. Precision of systematic sampling and transitive methods. J. Statist. Plan. Inf., 77, 263–279 (1999).
MATHERON, G., The Theory of Regionalized Variables and Its Applications. Les Cahiers du Centre de Morphologie Mathématique de Fontainebleau, No. 5. École Nationale Supérieure des Mines de Paris, Fontainebleau (1971).
MATHIEU, O., CRUZ-ORIVE, L.M., HOPPELER, H., WEIBEL, E.R., Measuring error and sampling variation in stereology: comparison of the efficiency of various methods for planar image analysis. J. Microsc., 121, 75–88 (1981).